Journal: Radiation research

Article Title: Dickkopf-1 Treatment Stimulates Hematopoietic Regenerative Function in Infused Endothelial Progenitor Cells

doi: 10.1667/RR15361.1

Figure Lengend Snippet: Dkk1-EPC transplantation increases hematopoietic stem cell content. Panel A: Top left side, representative FACS plots showing expression of c-Kit and Sca-1 within the BM lineage− (lin−) cell fraction of nonirradiated and 5 Gy irradiated mice at day 10. Top right side, quantification of the percentage c-Kit+Sca-1+lin− (KSL) cell population within the treatment groups. Lower left side, representative FACS plots showing expression of CD34 and Flt3 expression within the KSL gate. Lower right side, quantification of percentage CD34−Flt3− KSL HSCs (N = 10 mice/group; *P < 0.05 one-way ANOVA with multiple comparison test). Panel B: Mean KSL cells per femur over time. (N = 5–10 mice per group and time point; *P < 0.05 for significantly different treatment groups based on two-way ANOVA.) Panel C: CFC assay from day 10 treatment groups (N5 mice/group; P, 0.05 one-way ANOVA with multiple comparison test). Panel D: Survival after 8 Gy TBI: 15/15 Dkk1-EPC, 12/15 EPC and 5/15 saline. (N = 15 mice/group; P < 0.001 log-rank test).

Article Snippet: FACS Analysis Antibodies used for characterization of BM progenitor and HSC populations were purchased from BD Biosciences (San Jose, CA): Sca-1 (APC-CY7), c-Kit1 (PE) lineage cocktail (V450), CD34 (APC), Flt3 (FITC) or isotype controls.

Techniques: Transplantation Assay, Expressing, Irradiation

Journal: Frontiers in Immunology

Article Title: Specific Induction of Double Negative B Cells During Protective and Pathogenic Immune Responses

doi: 10.3389/fimmu.2020.606338

Figure Lengend Snippet: Distribution of B cell subsets in the peripheral blood of patients with neuro-inflammatory disorders or following vaccination with influenza or tick borne encephalitis virus. B cell subsets are presented as a percentage of total CD19 + B cells and defined as follows: naïve B cells CD19 + CD20 + CD27 - CD38 + IgD + , memory B cells CD19 + CD20 + CD27 + CD38 + , double negative B cells (DN B cells) CD19 + CD20l ow CD27 - IgD - , and plasmablasts CD19 + CD20 low CD27 + CD38 high IgD - . (A) Peripheral blood B cell subsets were quantified from patients with neuromyelitis optica spectrum disorder (NMOSD), myasthenia gravis, meningitis/encephalitis (mening./enceph.), Guillain-Barré syndrome (GBS), multiple sclerosis (MS), non-inflammatory neurological diseases (NIND) and patients with rheumatic diseases. Red dots highlight samples obtained from rituximab-treated patients, these data points were excluded from statistical analysis. Clinical groups were compared using a Kruskal-Wallis test with correction for multiple comparisons. Peripheral blood B cell subsets following vaccination against (B) influenza virus (n = 22) or (C) tick-borne encephalitis virus (n = 6). Statistical measurements were performed using a repeated measures ANOVA with Dunnett’s post-hoc correction. (D) Staining of different B cell populations was visualized by ImageStream. Lines in the graphs indicate median values and asterisks describe significance values as follows: *p < 0.05, **p < 0.01, ***p < 0.001, n.s. = not signifciant.

Article Snippet: For flow cytometric analyses, the following antibodies were used for all analyses: CD38 FITC (BD), IgD PE (Biozol), CD19 ECD (Beckman Coulter), CD3 PeCy7 (Beckman Coulter), CD45 VM (V450, BD), CD27 APC (BD), CD20 APC Cy7 (BD).

Techniques: Staining